Research progress in the association of peri-implant diseases and metabolic syndrome / 中华口腔医学杂志

Peri-implant disease, an important group of diseases that cause implant failure, are associated with metabolic abnormality. Metabolic syndrome (MetS) is a common metabolic disorder comprising abdominal obesity, hyperglycemia, systemic hypertension and atherogenic dyslipidemia. Previous studies had reported that MetS and its diversified clinical manifestations might be associated with peri-implant diseases, but the relationship and underlying mechanisms were unclear. This review aims to explore the relationship between MetS and peri-implant disease, in order to provide beneficial reference for the prevention and treatment of peri-implant disease in patients with MetS.

Clinical application of a quantitative method of atlantoaxial reduction angle in basilar invagination / 中华外科杂志

Objectives: To investigate the clinical application effect of a quantitative method of atlantoaxial reduction angle in basilar invagination. Methods: A retrospective analysis of clinical and radiographic data was conducted of 38 patients with complicated atlantoaxial dislocation and basilar invagination admitted to the Department of Neurosurgery, First Affiliated Hospital of Chongqing Medical University from May 2020 to May 2022. There were 5 males and 33 females, aged (53.5±9.9) years (range: 38 to 80 years). All patients underwent C1-2 interarticular fusion cage implantation+occipital-cervical fixation by pressing rob with the cantilever technique. The atlantoaxial reduction model of previous studies by our team was used to calculate the reduction angles before surgery. Then titanium rods of prebending angle were prepared according to the calculation before the operation. After that quantitative reduction of angle was performed during the operation. The paired t-test was used to compare the difference between the theoretical and actual reset value. Results: The theoretical reduction angle of all patients was (10.62±1.78)° (range: 6.40° to 13.20°), the actual reduction angle was (10.53±1.63)° (range: 6.70° to 13.30°) and there was no statistical difference between them (t=1.688, P=0.100). The theoretical posterior occipitocervical angle after the operation of all patients was (117.37±5.88)° (range: 107.00° to 133.00°), the actual posterior occipitocervical angle after the operation was (118.25±6.77)° (range: 105.40° to 135.80°) and there was no statistical difference between them (t=-0.737, P=0.466). The postoperative follow-up time of the patients was more than 6 months and the symptoms of all patients were relieved. All patients had satisfactory fusion between small joints without incision infection, internal fixation fracture, displacement, atlantoaxial redislocation, and other long-term complications. Conclusion: The quantitative method of atlantoaxial reduction angle in basilar invagination can calculate the theoretical reduction angle of the clivus axis angle and guide the preparation of the pre-bending titanium rod before surgery, so as to realize the quantification of the atlantoaxial reduction angle.

Comparison of nighttime and daytime operation on outcomes of kidney transplant with deceased donors: a retrospective analysis / 中华医学杂志(英文版)

BACKGROUND@#Kidney transplant is always emergent operations and frequently need to be performed at nighttime to reduce cold ischemia time (CIT). Previous studies have revealed that fatigue and sleep deprivation can result in adverse consequences of medical procedures. This study aimed to evaluate whether nighttime operation has adverse impact on kidney transplant.@*METHODS@#A retrospective analysis of recipients accepted kidney transplant from deceased donors in one center from 2014 to 2016 was performed. Daytime transplant was defined as operation started after 8 AM or ended before 8 PM and nighttime operation was defined as operation ended after 8 PM or started before 8 AM. The incidences of complications such as delayed graft function, acute rejection, surgical complications and nosocomial infections were compared between 2 groups. Student's t-test was used to analyze continuous variables such as serum creatinine (Scr) at 1-year of post-transplant. The Chi-square test was used to analyze categorical variables. Differences in recipients and graft survival were analyzed using Kaplan-Meier methodology and log-rank tests.@*RESULTS@#Among the 443 recipients, 233 (52.6%) were classified into the daytime group and the others 210 (47.4%) were in the nighttime group. The 1-year survival rate of recipients was similar for the recipients in the daytime and nighttime groups (95.3% vs. 95.2%, P = 0.981). Although the 1-year graft survival rate in the nighttime group was slightly superior to that in the daytime group, the difference was not significant (92.4% vs. 88.4%, P = 0.164). Furthermore, Scr and incidence of complications were also not significantly different between the 2 groups.@*CONCLUSIONS@#Our results suggested that operation time of kidney transplant with short CIT has no significant impact on the outcome of kidney transplant. Nighttime operation of kidney transplant with short CIT could be postponed to the following day to alleviate the burden on medical staffs and avoid the potential risk.

Clinical Effect of Modified Tongqiao Huoxuetang Combined with ‘Huiyang Jiuzhen’ in Treating Post-stroke Cognitive Impairment / 中国实验方剂学杂志

Objective: To discuss the clinical efficacy of modified Tongqiao Huoxuetang combined with ‘Huiyang Jiuzhen’ in treating post-stroke cognitive impairment (PSCI) and its mechanisms in resisting inflammation and oxidant stress and ameliorating hemorheology. Method: One hundred and twenty-two patients were randomly divided into control group (61 cases) and observation group (61 cases) by random number table. Both groups got Donepezil tablets, 10 mg/time, 1 time/day, and Nicergoline tablets, 20 mg/time, 3 times/days. Patients in control group got ‘Huiyang Jiuzhen’, 1 time/day, 6 times/week. In addition to the therapy in control group, patients in observation group got modified Tongqiao Huoxuetang, 1 dose/day. And a course of treatment was 8 weeks. Before treatment and at the 4th week and 8th week after treatment, scores of mini-mental state examination (MMSE) and Montreal cognitive assessment (MoCA) were discussed. And activity of daily living scale (ADL), syndrome of blood stasis and cerebral collaterals were graded. And levels of Interleukin-6 (IL-6) before and after treatment, C-reactive protein (CRP), tumor necrosis factor-α (TNF-α), oxidized low density lipoprotein (Ox-LDL), superoxide dismutase (SOD), malondialdehyde (MDA), hemorheology were detected. Result: According to the rank sum test, the clinical efficacy in observation group was better than that in control group (Z=1.976, Pth week and 8th week after treatment, score of MMSE and MoCA were higher than that in control group (PPPα, CRP, DA and Ox-LDL were lower than those in control group (PPPConclusion: In addition to the routine therapy of western medicine, modified Tongqiao Huoxuetang combined with ‘Huiyangjiuzhen’ can improve patients cognitive function and daily life ability, have effects in resisting inflammation and oxidation and ameliorating hemorheology, and can promote the recovery of cognitive function.

Predictive Score Model for Delayed Graft Function Based on Hypothermic Machine Perfusion Variables in Kidney Transplantation / 中华医学杂志(英文版)

Background@#Hypothermic machine perfusion (HMP) is being used more often in cardiac death kidney transplantation; however, the significance of assessing organ quality and predicting delayed graft function (DGF) by HMP parameters is still controversial. Therefore, we used a readily available HMP variable to design a scoring model that can identify the highest risk of DGF and provide the guidance and advice for organ allocation and DCD kidney assessment.@*Methods@#From September 1, 2012 to August 31, 2016, 366 qualified kidneys were randomly assigned to the development and validation cohorts in a 2:1 distribution. The HMP variables of the development cohort served as candidate univariate predictors for DGF. The independent predictors of DGF were identified by multivariate logistic regression analysis with a P < 0.05. According to the odds ratios (ORs) value, each HMP variable was assigned a weighted integer, and the sum of the integers indicated the total risk score for each kidney. The validation cohort was used to verify the accuracy and reliability of the scoring model.@*Results@#HMP duration (OR = 1.165, 95% confidence interval [CI]: 1.008-1.360, P = 0.043), resistance (OR = 2.190, 95% CI: 1.032-10.20, P < 0.001), and flow rate (OR = 0.931, 95% CI: 0.894-0.967, P = 0.011) were the independent predictors of identified DGF. The HMP predictive score ranged from 0 to 14, and there was a clear increase in the incidence of DGF, from the low predictive score group to the very high predictive score group. We formed four increasingly serious risk categories (scores 0-3, 4-7, 8-11, and 12-14) according to the frequency associated with the different risk scores of DGF. The HMP predictive score indicates good discriminative power with a c-statistic of 0.706 in the validation cohort, and it had significantly better prediction value for DGF compared to both terminal flow (P = 0.012) and resistance (P = 0.006).@*Conclusion@#The HMP predictive score is a good noninvasive tool for assessing the quality of DCD kidneys, and it is potentially useful for physicians in making optimal decisions about the organs donated.

The investigation of DNA double-strand breaks of lymphocytes in workers exposed to lead and DNA double-strand breaks of human lymphocytes induced by lead in vitro / 中华劳动卫生职业病杂志

<p><b>OBJECTIVE</b>To study DNA double-strand breaks of human peripheral lymphocytes exposed to lead with flow cytometry (FCM).</p><p><b>METHODS</b>The lymphocytes were obtained from 36 workers occupationally exposed to lead and 70 residents without occupational exposure to lead. DNA double-strand breaks were detected by flow cytometer assay. The lymphocytes from health people were incubated with lead at different doses and time, FCM assay was used to detect DNA double-strand breaks.</p><p><b>RESULTS</b>DNA double-strand breaks and fluorescence intensity of high exposed group and low exposed group were 41.76% ± 28.57%, 9.90 ± 3.35 and 33.18% ± 30.64%, 9.39 ± 4.83, respectively, which were significantly higher than those (0.28% ± 0.28% and 6.95 ± 2.93) of control group (P<0.05). The results of in vitro experiment indicated that DNA double-strand breaks of lymphocytes exposed to Pb at the dose of 125.0, 250.0, 500.0 µmol/L for 1 and 2 h were significantly different from those of the negative control group and positive control group (P<0.01). DNA double-strand breaks increased at beginning and then decreased with lead doses.</p><p><b>CONCLUSION</b>Lead can induce DNA double-strand breaks, γH2AX detected using flow cytometer assay can be used to measure the DSBs of DNA in large samples.</p>

The expression and significance of decorin in chronic rejection of liver transplantion / 国际外科学杂志

Objective To investigate expression and significance of decorin(DCN)in liver tissue and serum of liver transplant patients with chronic rejection(CR).Methods Immunohistochemistry(SP method)was used to detect expression of DCN in liver tissue of 16 normal controls, 20 patients with cirrhosis, 46 liver translantion patients without CR and 8 patients with CR.Enzyme-linked immunosorbent assay method(ELISA method)was used to determined the content of DCN in serum of all research subjects.Results The expression of DCN was negative in normal hepatic tissues and with/without CR, cirrhosis tissues showed strong expression of DCN.The positive expression rate and the average optical density value of DCN in liver transplant tissues with CR had significant difference comparing with Cirrhosis tissues(25% vs 55%, 0.1249 ±0.0039 vs 0.2357 ±0.0396, P <0.01,while no statistic siqnificance compared to normal liver tissues and those without CR.The level of DCN in serum was significantly higher in liver transplant patients with CR, with significant difference comparing with normal people, liver cirrhosis and transplant liver patients without CR(54.0833 ± 6.0325)μg/L vs(1.0232 ± 0.9105)μg/L,(12.6202 ± 1.5370)μg/L,(17.7102 ± 2.3562)μg/L, P < 0.01).The concentration of DCN in serum showed a positive correlation with the degree of CR.Conclusions DCN showed negative expression in liver tissue and increased significantly in serum of liver transplantation patients with CR.This suggests that DCN may be involved in occurrence and development of CR.At the same time the determination of DCN in serum maybe become an important indicator of the early diagnosis, development and prognosis of CR for liver transplant patients.

Detection of DNA damage of workers occupationally exposed to lead with flow cytometer assay / 中华劳动卫生职业病杂志

<p><b>OBJECTIVE</b>To study DNA damage of workers occupationally exposed to lead with flow cytometer assay.</p><p><b>METHODS</b>The lymphocytes were obtained from 41 workers occupationally exposed to lead (comparable group) and another 50 from control group. Flow cytometer (FCM) assay was used to detect DNA damage.</p><p><b>RESULTS</b>DNA damage rate and geometric mean fluorescence intensity in the comparable group were significantly higher than those in the control group (P<0.05). There were no significant differences in the DNA damage and geometric mean fluorescence intensity between different age groups (P>0.05). The differences in correlation analysis between blood lead, urine lead, delta-ALA and DNA damage rate were not significant (P>0.05). The correlation analysis showed no statistical significance between concentration of blood lead, urine lead, delta-ALA and geometric mean fluorescence intensity (P>0.05). There was positive correlations not only between the high concentration of blood lead, delta-ALA and damage rate of DNA, but also between the high concentration of blood lead and geometric mean fluorescence intensity. The coefficient r showed statistical significance (P<0.05).</p><p><b>CONCLUSION</b>Occupational lead exposure can cause DNA damage. Gamma H2AX flow cytometer assay is a sensitive, objective and effective method for detection of DNA damage of peripheral blood lymphocytes.</p>